The analysis of literature data revealed that three-months teaching of elderly and old people the principles of healthy life style significantly improve quality their life. If ageing people order the main principles of a healthy life style, it can make their life span 3-10 years longer, and this effect is the greater, the earlier they start to adhere to a healthy behavior. Graduated / moderate, prolonged constraint of caloric value of food (on 25 per cent from due value of energy consumption) can lower the biological age in ageing people up to about 7 years

It’s well-known that osteoarthritis (OA) is a widespread form of degenerative joint disorder that has the significant genetic component. The studies concerning the role of various candidate genes in the OA development are analyzed; much attention is given to the exploration of matrix metalloproteinases, their inhibitors and interleukin genes polymorphisms. The paper describes the results of up-to-date genome-wide association scanning and meta-analyses of polymorphisms associated with osteoarthritis in detail. The paper also considers the problem of the linkage between epigenetic alterations and the susceptibility to OA.

A review of the literature devoted to the topical issues of the treatment of portal hypertension depending on the form (extrahepatic, intra-hepatic), contains the definition, classification, etiology, pathogenesis and the main clinical manifestations of this disease. In detail, in chronological order, discussed the questions of conservative and surgical treatment, including endoscopic interventions, endovascular techniques portosystemic bypass surgery, treatment of complications: ascites, variceal bleeding, secondary immunodeficiency

Purpose: Compare efficacy and safety of Neo-Penotran Forte and Vaginorm C intravaginal pharmaceuticals in bacterial vaginosis treatment.
Materials and methods: 84 female patients were inspected at the age from 18 to 45 years old, 31,5 years old in average, with bacterial vaginosis diagnosis. 42 patients were treated with Neo-Penotran Forte, and 42 patients were treated with Vaginorm C. All the female patients were divided in two groups in accordance with the used treatment mode: the 1st group was treated with Neo-penotran forte (vaginal suppositories) (n=42), the 2nd group was treated with Vaginorm C (vaginal tablets) (n=42). Treatment efficacy and safety was evaluated 3 days after the treatment was over and a month after the treatment was over.
Results: The treatment with Neo-Penotran Forte showed more pronounced clinical and laboratory effect both at the first and the second visits in comparison with Vaginorm C.
Summary: The most effective scheme of bacterial vaginosis treatment is metronidazole and miconazole combination. Efficacy of metronidazole and miconazole combination treatment is 100%, efficacy of ascorbic acid treatment is 80%.

Purpose: To evaluate the clinical response and quality of life of patients with uterine myoma on the basis of the analysis of outcomes after organ-preserving interventions and depending on existence of complex rehabilitation.
Materials and Methods: This prospective study included 265 patients with symptomatic uterine myoma. Data on health-related quality of life and severity of symptoms before and after UAE and organ-preserving interventions and existence of complex rehabilitation were obtained by the standardized «Uterine Fibroid Symptom and Quality of Life» (UFS-QOL) questionnaire. Treatment failure was defined as the need for a second invasive procedure because of recurrent symptoms or persistent symptoms after interventions.
Results: At 6 months after treatment, all three leiomyoma therapies resulted in substantial symptom relief, to near normal levels, with the greatest improvement after complex rehabilitation.
Summary: It is shown that the assessment of quality of life not only a prediсtor of the complicated current of the postoperative period and the remote recurrence of a disease, but also criterion of full value of a carried-out course of rehabilitation. The complex rehabilitation makes it possible to reduce the frequency of postoperative complications and relapses of diseases.

Purpose: To research of certain peculiarities of the virus-microbial assotiations of middle ear in chronic suppurative otitis media (CSOM).
Materials and Methods: Microflora in the middle ear of 102 patients aged from 20 to 70 years with chronic suppurative otitis media were studied by microbiological and genetic (PCR) methods.
Results: Microbiotic mixed infection was detected in 62,5% patients and microbiotic monoinfection–in 37,5% patients. In microbiotic monoinfection the frequency of occurance of herpes and papilloma viruses was 19,4% and in microbiotic mixed infection - 32,2%.Chlamydiae were detected only in mixed infection (16,7%). Presence of mycoplasms in middle ear in microbiotic monoinfection was 13,7 times less (p<0,01) than in mixed infection.
Summary: The obtained results are allowed to recommend broadened microbiological analysis with application PCR to put into practice to increase the level of diagnostics and to make choice of an adequate treatment of CSOM.

Purpose: The study of operation diagnostic characteristics of a newly constructed helicobacter test-system which is designed to determine the total antibodies in the blood serum in volumetric agglomeration reaction.
Materials and methods: Blood sera of 224 gastroenterological patients were studied with the help of diagnostic system constructed. Commercial immune-enzyme blood assay systems were used to be the referent method.
Results: Diagnostic operation characteristics of a latex antigen helicobacter test-system were determined with the help of referent serological diagnostic methods. At the cut-off point 1:160 the sensitivity, specificity and diagnostic effectiveness are 94,9%, 79,3% и 88,2%, respectively.
Summary: The cut-off point 1:160 is shown to be used as a diagnostic threshold.

Purpose: Comparative analysis serologic screening techniques of epigenetic and colon cancer for adequate screening and diagnostic algorithms for detection of colorectal cancer.
Materials and methods: Surveyed 204 patients whose colon cancer was detected in screening activities. A control group consisted of 56 healthy people. Screening measures unite following techniques: Immunochromatographic fecal occult blood, determination of tumor markers in the blood (carcinoembryonic antigen, oncoantigens CA 19-9, CA 242), methylated DNA gene septins 9 in the blood. The ROC-analysis determined the significance of diagnostic tests and their aggregation.
Results: Diagnostic efficacy of colon cancer screening by blood levels of CEA, CA 242 and CA 19-9, respectively, 40,4%, 50,8%, 51,7%. Epigenetic testmetilat DNASEPT9of blood as screening method is characterized by a high diagnostic sensitivity-85,3%.
Summary: At the initial stage of colon cancer screening method is a combination of the optimal combination of immunochemical test for the occult blood in feces in the threshold of 100 ng/ml, metilat DNA detection of SEPT9 gene in the blood.

Purpose: The study aims to improve the effectiveness of surgical treatment of rectocele by developing and implementing an original method of operation.
Materials and methods: A comprehensive analysis of the survey results and surgical treatment of 32 patients with rectocele. In 12 patients used the methods sfinkterolevatoroplastik front, and 8 - endorectal hardware circular mucosal-submucosal resection (using Longo).
Results: The resulting clinical experience has allowed to develop an original method of combined surgical treatment of rectocele, combining the technology of circular endorectal mucosal-submucosal resection and front levatoroplastik. The proposed method is aimed at strengthening the rectovaginal septum, the restoration of its interior and the creation of the holding fascial-muscular frame. The method was tested in 12 patients with rectocele II and III.
Summary: The application of the developed method, which takes into account the main elements of the pathogenesis of rectocele, achieves stable positive results, and reduces the likelihood of recurrence of the disease.

Purpose: To evaluate the clinical clinical application subtenon administration of bevacizumab on the viscous media with the purpose of strengthening and prolong of therapeutic effect in the treatment of wet form of age-related macular degeneration (AMD).
Materials and Methods: The study included 6 patients (6 eyes) with the wet form of AMD, who underwent the introduction of bevacizumab at a dose of 5 mg (0.2 ml) into the back subtenon space for viscous media – hydroxypropylmethylcellulose (the patent application on the invention of the Russian Federation №2013105697 from 11.02.2013). All patients underwent visometry, biomicroscopy, ophthalmoscopy, fundus photographic recording, optical coherence tomography and fluorescein angiography. The follow-up 3 months.
Results: The mean values of visual acuity with correction improved after treatment (from 0.06 to 0.2). Central retinal thickness average decreased after treatment all patients (363.5 μm to 309 μm). Also, we have seen a significant decrease in transsudativnyh processes in the retina in all patients and reducing the suretinal of a neovascular membrane into 1.2 times, that speaks about supperession of activity choroidal neovascularization. The effect was observed throughout the period of observation.
Summary: Subtenon introduction of bevacizumab is safe and has a positive effect in wet AMD, and its application to a viscous mediа has a prolonged effect.

Purpose: Development of methods of sedation with preserved consciousness in carotid endarterectomy under regional anesthesia and a way to monitor the depth of sedation using scales degree of sedation and indicators BIS monitoring.
Materials and Methods: The study included patients to whom performance of CEA was planned under regional anesthesia without the installation of an intraluminal shunt. CEA was performed to 72 patients aged 50 to 80 years. After performing the regional block, we started infusion of propofol target concentration using diprifusor TCI system.
Results: During anesthesia, we have allocated five stages, each of which required a level of sedation. The Dependence of BIS index and the degree of sedation RAMSAY from propofol TCI, allows quantitatively assess the degree of sedation and individually select the desired level of sedation. As a result, during cross-clamping of the ICA patients can actively cooperate with the anesthetist for dynamic neurological control.
Summary: The operation CEA under sedation with preserved consciousness allows to use all the advantages of regional anesthesia, creating fairly comfortable environments for patients. Rare cases of respiratory depression, sedation allows the independent external patient`s breathing. That allows dynamic neurological control and to maintain constant verbal contact.

Purpose: Examine the state of cerebral hemodynamics in patients with obliterative atherosclerosis of the lower extremities.
Materials and methods: Patients were divided into 3 groups depending on the degree of chronic limb ischemia. In one group (67 people) included patients with B II degree of ischemia. The 2nd group (36 patients) included patients with grade III ischemia. In the 3rd group entered 21 patients with a decompensation of collateral blood circulation into the extremities, being accompanied the expressed pain syndrome (the IV degree of ischemia). State of the blood flow was assessed by Doppler ultrasound data on the levels of the extracranial and intracranial arteries brachycephalic departments.
Results: In patients with decompensation of collateral circulation of the lower limbs (group 3), a significant decrease in the rate of blood flow in the internal carotid arteries in comparison with the indicators of all other groups .
Summary: Severe chronic ischemia of the lower limbs should be seen not only as a negative factor for the lower extremities, but also as a factor in reducing the risk of cerebral perfusion and significant deterioration of autoregulatory reserve of cerebral hemodynamics.

Purpose: To study the structural changes of the architectonics of renal microvasculature in cardiovascular diseases.
Materials and methods: The investigation has been performed in 178 kidneys of human corpses of various ages. 110 of them died from some cardiovascular diseases (atherosclerosis of the abdominal aorta, hypertension, chronic ischemic cardiac disease and their combinations) and 68 from mechanical asphyxia. Macro-microscopical, histological, biometrical methods and scanning electron microscopy have been used.
Results: We revealed following microcirculatory bed’s changes: juxtamedullary shunting, excessive hyperemia and considerable deterioration of blood supplay both the renal cortical and medullary parts.
Summary: The greatest changes in architectonics of microcirculatory bed in kidneys were observed at hypertension combined with chronic cardiac ischemia, and the least ones- at atherosclerosis.

Purpose: assessment of activity of free radical oxidation and antioxidant protection during vitrectomy in patients with retinal detachment.
Material and methods. We examined 67 patients with retinal detachment of various etiologies who underwentvitrectomy.
Results. We observed a positive dynamics of free radical oxidation after the operation - a full recovery of biochemical parameters in the late postoperative period. Among the complications mentioned cataract development in some patients who did not receive antioxidant therapy.
Summary: Optimal from the point of view of both the functional results and the status of free radical oxidation and antioxidant protection is the additional use during surgery means of antioxidant protection.

Purpose: To evaluate the squamous epithelialization in Barrett’s esophageal mucosa after forming arefluх cardia.
Materials and Methods: A dynamical endoscopical examination of 47 patients with Barrett’s esophagus after forming arefluх cardia followed by taken biopsies for morfohistochemical and immunohistochemical study.
Results: After surgical treatment in the mucosa of Barrett’s esophagus foci of squamous epithelialization are formed.
Summary: Formed areflux cardia prevents reflux of acidic stomach contents into the esophagus, that contributes the regeneration and epithelialization of the mucosa.

Purpose: Improvement of results of treatment of patients with the intra hepatic portal hypertensia (PH) a way of performance of a decompression of portal system installation of the intra hepatic shunt.
Materials and methods: The group of supervision was made by 128 patients from intra hepatic PH aged from 14 till 83 years: 77 men (60,2%) and 51 women (39,8%) by which PH for the purpose of correction was executed the TIPS.
Results: TIPS provides a decompression of portal system in 100%. The lethality made 2,34%. One of patients was lost as a result of yatrogenny injury of a vorotny vein, two patients with chronic hepatocellular insufficiency «C» a class died of the phenomena of the hepatokidney insufficiency aggravated with executed intervention.
Summary: TIPS is optimum for heavy category of patients with cirrhosis, is shown to patients at height of varicose bleeding, can be combined from an embolizatsiy left gastric vein, without complicating shunting procedure.

Purpose: Analysis of frequency, localization, morphological characteristics, policy of colorectal cancer treatment.
Materials and methods: 745 medical histories of patients admitted with colorectal cancer from 2001 till 2011 were studied in the surgical department of Rostov State Medical University.
Results and Summary: It was determined that cancer of rectum and sigmoid colon were founded mostly among women at the age of 61 – 70 years old. The most common was the third stage of the disease and endophytic type of growth. It was noted that the moderately differentiated adenocarcinoma was the mostly widespread. Extirpation of rectum with resection of the segment Sigma; anterior resection of the rectum with hardware colorectal anastomosis end-to-end were used in most cases for colorectal cancer.

Purpose: Construction of a personal relevant database on spectra of V. cholerae strains belonging to different biotypes, serological groups and toxigenic levels, which will allow for the identification, differentiation and comparison of bacterial isolates on the base of analysis of V. cholerae ribosomal protein cell fingerprints.
Materials and methods: For the construction of the reference library of mass charges m/z 100 passportized strains from museum collection have been used, characterized by the complex of parameters, namely: by serogroup, biotype, object and year of isolation and by the presence of cholera toxin genes (ctxAB+).
Results: As the result of the studies performed the personal database has been developed for the expanded collection of Vibrio cholerae protein spectra in addition to MALDI Biotyper (“Bruker Daltonics”) (Certificate of State Registration for database “Proteomic Profiles of Mass-Spectra of Strains Representing V. cholerae Species for MALDI Biotyper Program” No. 2013620585 of 29.04.2013).
Summary: The relevant protein spectra database developed provides the possibility to perform interspecies and partial intraspecies differentiation of V. cholerae О1 and О139 strains on the base of analysis of fingerprints of V. cholerae ribosomal protein cells.

Purpose: study of the morphofunctional peculiarities of students on the medical faculty/
Materials and methods: 62 students of 1st course, studying in the main group of physical education were surveyed. 578 medical control cards of graduates year 2007 – 2012 were analysed. During the survey the following methods were used: Body Type, Artenal Blood Pressure, Pulsometry, ECG diagnostics, Spirography, Stepergometry according to location PWC 170. Calculation method: the adaptive capacity, physical efficiency, MOC (maximum oxygen consumption) and AP (aerobic performance) were calculated.
Results: 36% of the students of 1st course in the main physical education group was related to the MaC and MeMaC – types with resistive mechanism of adaptation. 28,5% of female and 32,7% of male graduates had decreased physical efficiency and indicators of life – support.
Summary: students of the main group of physical education need to have differentiated programs of physical training, given that the adaptive capacity and body type are taken in to consideration. To determine the individual tolerance to physical stress, 60% of the graduates will need to undergo veloergometry with pulse, blood pressure and ECG being registered and cotrolled.

Purpose: Iatrogenic bile duct injuries (BDI) is an important problem in abdominal surgery. According to the literature, different methods of surgical reconstruction are performed in patients with BDI. The most frequent procedure is the Roux-en-Y hepaticojejunostomy (HJS) which recommended by most authors.
Materials and Methods: 66 patients with BDI were admitted to our hospital and operated on in period of 1997-2013. Primary end-to-end ductal repair was used in 2 cases in 1-2 days after injury without T-tube drainage and in the third case 5 day after the accident using forenamed drainage. Successful right hemihepatectomy was performed in 1 patient; in 62 patients - Roux-en-Y HJS, 23 among them using our antireflux modification of the anastomosis (Russian Fed. Patent N 2470592).
Results: There were the following criteria for research: wound infection, cholangitis attacks, anastomotic leak, period of time for postoperative blood- and liver function analyses restitution. Total 30-days mortality was 1,5% (1 patient). Mortality wasn’t registered in the group of antireflux interventions. Estimation of follow-up results showed the advantages of the proposal method.
Summary: The method of choice in surgery of iatrogenic BDI and subsequent biliary ductal obstruction due to BDI is repair using antireflux Roux-en-Y HJS.

Purpose: To improve coloesophagoplasty outcomes in children with esophageal atresia when diastasis between the colon and neck part of esophagus came to 3.0 cm by lengthening the last one.

Materials and Methods: A new method of esophagomyotomy using only longitudinal muscle fibers dissection was developed experimentally. This method was applied to perform an esophagoplasty in 3 children with primary-made double esophagostomy. The “end-to-end” esophagocoloanastomosis and presternum resections were made in all cases.

Results: Keeping the circulatory muscle layer intact allows to avoid submucosal vessel’s damage, esophageal diverticula’s formation and to facilitate surgeon’s work, to decrease a period of operation. Two neck anastomoses were healed without fistula’s formation, one girl developed anastomosis inconsistency, so separation of anastomosis was made for a 6-month period with followed-up raconsruction.

Summary: The neck part of esophagus elongation using Livaditis-Kimura esophagomyotomy in cases of coloesophagoplasty in children with esophageal atresia can be used as alternative method to eliminate diastasis between ends of colon and esophagus up to 3.0 cm.

Purpose: To improve an artificial esophagus function using antireflux properties of modificated Depage-Janeway tube gactrostoma.

Materials and methods: 3 variants of cologastric anastomoses using such a gastric tube were developed and applied in 22 children: “end-to-end” (2), “end of gastric tube - to the colon side” (2) and “colon end - to the gastric tube side” (18).Results: The most acceptable food-passing properties of artificial esophagus were achieved using the third variant of anastomosis. It caused by a wide lumen of anastomosis, which was the same as colon’s graft lumen. After 0.5-1 year since the operation an X-ray examinations were performed and validated a satisfactory antireflux properties of gastric tube as a part of artificial esophagus.

Summary: Not the direct colo-gastric anastomosis, but using the gastric tube Depage-Janeway gastrostoma is the new method to solve the problem of antireflux protection of the graft during esophagoplasty in cases of scar strictures in children

Purpose: to support the use of hypoxic gas media for emergency restoration of functionality of the body specialists hazardous occupations.Materials and methods. The studies included 26 specialists hazardous occupations (men aged 20-32 years old), with signs of disadaptation. The 14 people (basic group - BG) conducted training with the use of normobaric hypoxic gas media - NHGM (O2 content 15-17%, the duration of each treatment 45 min, the total number of procedures - 12) starting from 6-th day of the hypoxic exposure combined with physical exercise. The remaining 12 people (comparison group - CG) performed a similar exercise to simulate the conditions of NHGM. To assess the functionality of the organism (FO) used the trial with stepwise increased physical load on the bike until the anaerobic threshold (AT), recording parameters of hemodynamics and gas exchange. Trial run for 1 day prior to the start of training courses and the day after.The Results. All persons of BG after the workouts recorded an increase in work load up to AT least 1 upper the baseline, in the absence of such changes to the CG, which revealed a statistically significant (p < 0.05) differences in direct indicators of physical performance (maximum power and load time). Persons of BG also noted a significant improvement of physiological, physical work: reduction in blood pressure and heart rate during load, improve maximal oxygen consumption. Such changes in persons of CG were absent.Summary. The program, based on the use of NHGM can be considered as a selection method in the physiological support of emergency restoration FO of specialists hazardous occupations.

Purpose: clinical and experimental study in the diagnosis of ophthalmic forms and severity of diverticular disease Physiological and pathological research methods conducted.Materials and Methods: according to the original method (Russian Federation patent № 2087943) a model of diverticulosis of the colon was applied to 40 animals (rabbits). Animals were divided into 4 groups.At the clinic were examined 64 patients with diverticular disease, without comorbidity: local diverticulosis (17.2%), subtotal (57.7%), total (9.4%), and chronic complications (18.7%).All patients took a biochemical test (Russian Federation patent № 2119168)Results: in diverticular disease of the colon in 100% of cases, violations of the visual analyzer are detected, due to violation of local hemodynamics, as well as the activation of the lipid peroxidation (LPO).Proved the effectiveness of ophthalmic examination in the diagnosis of patients with forms of diverticular disease and especially its chronic complications.Summary: the changes of the eyeball identified by experimental and clinical research are typical only for diverticular disease and reflect the severity of its course.

The article represents the epidemiological characteristics of the cholera outbreak, which originated in Mariupol, Donetsk region (Ukraine) in May and August 2011. Listed the major factors of transmission, causes, contributing to its spread. Outlined the results of laboratory tests of material from patients and objects in the environment. Highlighted the set of epidemic control activities on the localization of cholera aimed at each of the three units of the epidemic process. Defined priority actions for the period after the elimination of epidemic diseases to prevent complications in the future.

Results of treatment of two groups of patients suffering from oily seborrhea. Patients in both groups received traditional system and outside therapy. In the second group was used as a balm «Aysida» to strengthen the hair with oily seborrhea. The obtained results allow to achieve high efficiency, good tolerance and level side effects in most patients. During the period of remission, the use of this tool as a single agent is the prevention of future exacerbations.

The idea of development and self-development of subjectivity permeates the National doctrine of education in the Russian Federation up to 2025. Stresses the need for the mobilization of personal potential, integrity preservation, reproduction in every person’s quality of subjectivity. The challenge of education is to create the condition for gaining medical student personal meaning, values and goals of its development. The real result of education in these conditions is the development of the ability of medical students (future professional) to feel the way in changing the world and oneself as a part of this world, the willingness to meet with surprises and ability to respond to these meetings a positive innovation.

One of the major problems of patients who are inconditions of debilitating disease is personal self-determination in relation to the social status of disabled. Multi plein stances of unwarranted appeals patient expert solutions for the with drawal of disability, discrep ancies establishing group expectations of the patient, or primary failure to obtain group, demonstrate the relevance of this question, especially given the working-age people claiming disability, rehabilitation which (and their integration into the wider context of social and professional relationship) is the strategic direction of social policy. This article discusses there result personal response repertoire of coping strategies and the subjective experience of social frustration of patients who are fixed on a refund, establishing, strengthening the group, and patients who have similar disease, but do not consider themselves disabled.

In this article we show state homeopathic care in Saint-Petersburg. Study pattern and organization homeopathic care for children in leading homeopathic clinic of Saint-Petersburg and also applying for homeopathic care among the child population Saint-Petersburg. The analysis of morbility characters based on applying for homeopathic care among the child population has been performed in dynamics in the Saint-Petersburg during 3 year period from 2007 to 2009 on the basic received 1989 final (exacted) diagnoses in Saint-Petersburg’s homeopathic polyclinic.

The analysis of 5 cases of carcinoid papillomatosis of the skin Gottron that developed against the background of previous chronic dermatoses. In 2 patients with this pathology is combined with cancer of the internal organs.