Potential markers of obesity linked to the gut microbiome
https://doi.org/10.21886/2219-8075-2025-16-3-67-74
Abstract
Objective: to study the features of the content of some types of microorganisms in the colon in obese patients and healthy individuals with normal body weight using metagenomic sequencing. Materials and methods: a total of 265 people (44 men and 221 women, average age 47.1±4.8 years) were examined. Two clinical groups were formed: healthy individuals with normal body weight (n=129) and obese patients (n=136). The composition of the intestinal microbiome was assessed using metagenomic analysis. DNA was isolated from fecal samples and sequencing of the v3-v4 variable region of the 16S rRNA gene was performed. Results: statistically significant (p<0.05) differences in the quantitative and qualitative parameters of some types of microorganisms in the colon of healthy individuals without obesity and in obese patients were revealed. The number of P. copri was increased in obese patients. and decreased F. prausnitzii, B. adolescentis and B. longum. Conclusions: the obtained results demonstrated certain species differences in the intestinal microbiome in healthy individuals and in obese patients.
Supporting agencies: The work was carried out within the framework of the contract No. 0373100122119000041 for the project “Creation of a bank of blood serum and feces biosamples from healthy donors and patients with obesity, metabolic syndrome, type 2 diabetes mellitus, and impaired mucosal barrier of the gastrointestinal tract in order to identify candidate species-non- specific mediators of human microbiota quorum sensing systems that modulate the endocrine and metabolic function of adipose tissue”.
About the Authors
L. A. Ganenko
Rostov State Medical University
Russian Federation
Competing Interests:
Russian Federation
Lilia A. Ganenko, Cand. Sci. (Med.), Associate Professor of the Department of Internal Medicine N 3
Rostov-on-Don
Competing Interests:
Authors declares no conflict of interest
N. I. Volkova
Rostov State Medical University
Russian Federation
Competing Interests:
Russian Federation
Natalya I. Volkova, Dr. Sci. (Med.), Professor, Head of Department of internal diseases No 3
Rostov-on-Don
Competing Interests:
Authors declares no conflict of interest
A. V. Shestopalov
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
Russian Federation
Competing Interests:
Russian Federation
Alexander V. Shestopalov, D. Sci. (Med.), Professor, Deputy director, Center of Digital and Translational Biomedicine, Center for Molecular Health; Director, Department of postgraduate education, residency, postgraduate studies, Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology; Head, Department of biochemistry and molecular biology, Medical faculty, N.I. Pirogov Russian National Research Medical University
Moscow
Competing Interests:
Authors declares no conflict of interest
Yu. L. Naboka
Rostov State Medical University
Russian Federation
Competing Interests:
Russian Federation
Yulia L. Naboka, D. Sci. (Med.), Professor, Head, Department of microbiology and virology N 1
Rostov-on-Don
Competing Interests:
Authors declares no conflict of interest
A. L. Zibarev
Rostov State Medical University
Russian Federation
Competing Interests:
Russian Federation
Alexander L. Zibarev, Assistant Professor of the Department of Internal Medicine N 3
Rostov-on-Don
Competing Interests:
Authors declares no conflict of interest
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For citations:
Ganenko L.A., Volkova N.I., Shestopalov A.V., Naboka Yu.L., Zibarev A.L. Potential markers of obesity linked to the gut microbiome. Medical Herald of the South of Russia. 2025;16(3):67-74. (In Russ.) https://doi.org/10.21886/2219-8075-2025-16-3-67-74
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