Возможные маркеры ожирения, связанные с микробиомом кишечника

Цель: изучить особенности содержания некоторых видов микроорганизмов в толстой кишке методом метагеномного секвенирования у пациентов с ожирением и у здоровых людей с нормальной массой тела.

Материалы и методы: обследованы 265 человек (44 мужчин и 221 женщина, средний возраст — 47,1 ± 4,8 года). Сформированы две клинические группы: здоровые с нормальной массой тела (n = 129) и пациенты с ожирением (n = 136). Оценка состава микробиома кишечника выполнена путём метагеномного анализа. Из образцов кала выделяли ДНК и проводили секвенирование вариабельного участка v3–v4 гена 16S рРНК.

Результаты: выявлены статистически значимые (p < 0,05) различия количественных и качественных показателей некоторых видов микроорганизмов в толстой кишке у здоровых людей без ожирения и у пациентов с ожирением. У пациентов с ожирением повышено количество P. copri. и снижено количество F. prausnitzii, B. adolescentis и B. longum.

Выводы: полученные результаты продемонстрировали определённые видовые различия в микробиоме кишечника у здоровых лиц и у пациентов с ожирением.

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