MALDI MASS-SPECTROMETRIC ANALYSIS FOR TYPING AND INTRASPECIES DIFFERENTIATION OF CHOLERA VIBRIOS ON THE BASE OF CONSTRUCTION OF THE REFERENCE LIBRARY OF PROTEOMIC PROFILES
https://doi.org/10.21886/2219-8075-2014-2-88-91
Abstract
Purpose: Construction of a personal relevant database on spectra of V. cholerae strains belonging to different biotypes, serological groups and toxigenic levels, which will allow for the identification, differentiation and comparison of bacterial isolates on the base of analysis of V. cholerae ribosomal protein cell fingerprints.
Materials and methods: For the construction of the reference library of mass charges m/z 100 passportized strains from museum collection have been used, characterized by the complex of parameters, namely: by serogroup, biotype, object and year of isolation and by the presence of cholera toxin genes (ctxAB+).
Results: As the result of the studies performed the personal database has been developed for the expanded collection of Vibrio cholerae protein spectra in addition to MALDI Biotyper (“Bruker Daltonics”) (Certificate of State Registration for database “Proteomic Profiles of Mass-Spectra of Strains Representing V. cholerae Species for MALDI Biotyper Program” No. 2013620585 of 29.04.2013).
Summary: The relevant protein spectra database developed provides the possibility to perform interspecies and partial intraspecies differentiation of V. cholerae О1 and О139 strains on the base of analysis of fingerprints of V. cholerae ribosomal protein cells.
Materials and methods: For the construction of the reference library of mass charges m/z 100 passportized strains from museum collection have been used, characterized by the complex of parameters, namely: by serogroup, biotype, object and year of isolation and by the presence of cholera toxin genes (ctxAB+).
Results: As the result of the studies performed the personal database has been developed for the expanded collection of Vibrio cholerae protein spectra in addition to MALDI Biotyper (“Bruker Daltonics”) (Certificate of State Registration for database “Proteomic Profiles of Mass-Spectra of Strains Representing V. cholerae Species for MALDI Biotyper Program” No. 2013620585 of 29.04.2013).
Summary: The relevant protein spectra database developed provides the possibility to perform interspecies and partial intraspecies differentiation of V. cholerae О1 and О139 strains on the base of analysis of fingerprints of V. cholerae ribosomal protein cells.
About the Authors
N. R. Telesmanich
Control Plague Research Institute
Russian Federation
Russian Federation
V. V. Agafonova
Control Plague Research Institute
Russian Federation
Russian Federation
I. A. Chaika
Control Plague Research Institute
Russian Federation
Russian Federation
S. O. Seina
Control Plague Research Institute
Russian Federation
Russian Federation
O. S. Chemisova
Control Plague Research Institute
Russian Federation
Russian Federation
Ye. V. Goncharenko
Control Plague Research Institute
Russian Federation
Russian Federation
E. A. Menshikova
Control Plague Research Institute
Russian Federation
Russian Federation
M. V. Poleeva
Control Plague Research Institute
Russian Federation
Russian Federation
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Review
For citations:
Telesmanich N.R., Agafonova V.V., Chaika I.A., Seina S.O., Chemisova O.S., Goncharenko Ye.V., Menshikova E.A., Poleeva M.V. MALDI MASS-SPECTROMETRIC ANALYSIS FOR TYPING AND INTRASPECIES DIFFERENTIATION OF CHOLERA VIBRIOS ON THE BASE OF CONSTRUCTION OF THE REFERENCE LIBRARY OF PROTEOMIC PROFILES. Medical Herald of the South of Russia. 2014;(2):88-91. (In Russ.) https://doi.org/10.21886/2219-8075-2014-2-88-91
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