PREVENTION OF BACTERIAL COMPLICATIONS CORRECTION APOPTOSIS OF T-LYMPHOCYTES IN NEWBORNS WITH RESPIRATORY DISEASE
https://doi.org/10.21886/2219-8075-2013-4-134-138
Abstract
Purpose: prevention of bacterial complications correction apoptosis of T lymphocytes with human recombinant granulocyte colony-stimulating factor (G-CSF-par).
Materials and Methods: In randomized, controlled, blind clinical study included 69 neonates with respiratory pathology on mechanical ventilation who were born at term 39,2 ± 1,1 weeks, with a mass of 3650 ± 421, Apgar score 2,3±1,1, no clinical signs of infection, containing lymphocytes in early (AnnexinV-FITC + PI-) and late (AnnexinV-FITC + PI +) apoptosis than 9.59% and 0.56% (respectively) distributed in groups: patients I ( n = 39) received G-CSF is a par-10 mg / kg / day intravenously for the first 3 days, in group II (n = 30) par-G-CSF was not given. On admission, for 3-5 days and in the outcome of the disease by ELISA studied the content of G-CSF, FGF, sFas-L; subpopulations of lymphocytes by flow cytometry on a flow cytometer, Beckman Coulter Epics XL (USA).
Results: Statistical analysis of the results confirmed the reduction of the relative amount of lymphocytes activated Fas-receptor (CD95) and lymphocytes repositories early and late apoptosis, increasing stem cells in peripheral blood, elevated levels of FGF and antiapoptogennogo sFAS after administration par-GCSF, decrease the incidence of septic complications.
Summary: Timely correction of apoptosis of T-lymphocytes par-G-CSF is effective and safe profilaktiruet onset sepsis in neonates with respiratory disorders who are on mechanical ventilation.
About the Authors
M. G. Puchtinskaya
Rostov Scientific and Research Institute of Obstetrics and Pediatrics
Russian Federation
43, Mechnikova Str., Rostov-on-Don, 344012
Russian Federation
43, Mechnikova Str., Rostov-on-Don, 344012
V. V. Estrin
Rostov Scientific and Research Institute of Obstetrics and Pediatrics
Russian Federation
43, Mechnikova Str., Rostov-on-Don, 344012
Russian Federation
43, Mechnikova Str., Rostov-on-Don, 344012
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Review
For citations:
Puchtinskaya M.G., Estrin V.V. PREVENTION OF BACTERIAL COMPLICATIONS CORRECTION APOPTOSIS OF T-LYMPHOCYTES IN NEWBORNS WITH RESPIRATORY DISEASE. Medical Herald of the South of Russia. 2013;(4):134-138. (In Russ.) https://doi.org/10.21886/2219-8075-2013-4-134-138
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