The role of MMP and TIMP genetic polymorphisms in genital prolapse genesis

Abstract

Objective: to determine the association between MMP and TIMP genetic polymorphisms and GP risk. Materials and methods: Th e study involved 178 women aged 35 to 65, 134 of them with GP relapses (aft er hysterectomy by vaginal access because of a total and partial uterus and vaginal walls prolapse). Patients were randomized into the following groups: I – with manifestations of undiff erentiated connective tissue dysplasia (CTD)(11.7 points on average)(n = 86); II – with no CTD signs (n = 48). Control group III consisted of healthy women without any GP signs (n = 44). Used: genotyping by polymerase chain reaction of MMP / TIMP polymorphisms with separation of DNA samples from whole blood. Results: Statistically signifi cant diff erences were revealed in the distribution of polymorphisms frequencies in groups with GP and CTD signs compared to healthy women: MMP9 (rs3918242), ММР9 (rs17576); ММP3 (rs3025058); ММР2 (rs2285053)(rs2285052). Th e probability of pelvic fl oor failure increased while identifying genotypes: СТ gene MMP9 (OR=3,2; 95% CI 1,3-7,6), AG gene ММР9 (OR=2,9; 95% CI 1,2-7,0); 5A6A gene ММP3 (OR=3,7; 95% CI 1,3-10,1); СТ gene ММР2 (OR=3,2; 95% CI 1,3-7,5). Conclusion: Identifi cation of genetic predictors of pelvic fl oor remodeling with the formation of its insolvency contribute to better understanding of the pathogenesis of the disease and allow to stratify women into risk groups of GP development, progression or recurrence aft er surgery.