Adaptive immune response in patients with coronary artery calcification

Objective: to study and evaluate the state of adaptive immune response in patients with various coronary indices. Materials and methods: 62 male respondents of comparable age and risk factors for atherosclerosis participated in the study. Of these, 20 people with coronary atherosclerosis were in the moderate–risk group (group 1), 20 people with coronary atherosclerosis were in the high–risk group (group 2), and 22 people were in the control group. The assessment of coronary calcium and the calculation of the Agatston index were carried out using the software application for calculating coronary calcium Smart Score. The following CD3+, CD3+CD4+, and CD3+CD8+ populations were phenotyped with the calculation of the immunoregulatory index. B-lymphocytes (CD19+) were examined using flow cytofluorometry using appropriate monoclonal antibodies. Quantitative determination of the level of Ig A, M, G was carried out by radial immunodiffusion in gel using sets of monospecific antisera, expressed in g/L. Statistical analysis of the results of the study was carried out using the Statistica 12.0 program (StatSoft, USA). Results: in patients with coronary artery calcification, changes were detected in both the cellular and humoral components of adaptive immunity. Conclusion: these changes indicate the activation of the cytotoxic potential of CD8+ lymphocytes and the functional activity of B cells, which is the basis for further studies of the immune response in patients with coronary calcification, followed by the development of a universal screening tool.

1. Bogachevskaia S.A., Kiselev S.N. Analysis of cardiovascular mortality in Russia and the Far Eastern district in the framework of the implementation of the federal and regional programs «Fight against cardiovascular diseases»: targets, prognosis and facts. Far Eastern medical journal. 2024;(1):44-50. (In Russ.) http://doi.org/10.35177/1994-5191-2024-1-8

2. Zhuravlev K.N., Vasilieva E.Yu., Sinitsyn V.E., Spector A.V. Calcium score as a screening method for cardiovascular disease diagnosis. Russian Journal of Cardiology. 2019;(12):153-161. (In Russ.) https://doi.org/10.15829/1560-4071-2019-12-153-161

3. Miteva K, Madonna R, De Caterina R, Van Linthout S. Innate and adaptive immunity in atherosclerosis. Vascul Pharmacol. 2018:S1537-1891(17)30464-0. https://doi.org/10.1016/j.vph.2018.04.006. Epub ahead of print.

4. Agatston AS, Janowitz WR, Hildner FJ, Zusmer NR, Viamonte M Jr, Detrano R. Quantification of coronary artery calcium using ultrafast computed tomography. J Am Coll Cardiol. 1990;15(4):827-832. https://doi.org/10.1016/0735-1097(90)90282-t

5. Detrano R, Guerci AD, Carr JJ, Bild DE, Burke G, et al. Coronary calcium as a predictor of coronary events in four racial or ethnic groups. N Engl J Med. 2008;358(13):1336-1345. https://doi.org/10.1056/NEJMoa072100

6. Erbel R, Möhlenkamp S, Moebus S, Schmermund A, Lehmann N, et al. Coronary risk stratification, discrimination, and reclassification improvement based on quantification of subclinical coronary atherosclerosis: the Heinz Nixdorf Recall study. J Am Coll Cardiol. 2010;56(17):1397-1406. https://doi.org/10.1016/j.jacc.2010.06.030

7. Elias-Smale SE, Proença RV, Koller MT, Kavousi M, van Rooij FJ, et al. Coronary calcium score improves classification of coronary heart disease risk in the elderly: the Rotterdam study. J Am Coll Cardiol. 2010;56(17):1407-1414. https://doi.org/10.1016/j.jacc.2010.06.029

8. Safaryan A.S., Vygodin V.A., Nebieridze D.V., Nikonova K.V. Comparability of cardiovascular risk assessment according to the SCORE and the coronary artery calcium score (Agatston score). Cardiovascular Therapy and Prevention. 2023;22(9):3650. (In Russ.) https://doi.org/10.15829/1728-8800-2023-3650

9. Onnis C, Virmani R, Kawai K, Nardi V, Lerman A, et al. Coronary Artery Calcification: Current Concepts and Clinical Implications. Circulation. 2024;149(3):251-266. https://doi.org/10.1161/CIRCULATIONAHA.123.065657

10. Farias-Itao DS, Pasqualucci CA, Nishizawa A, da Silva LFF, Campos FM, et al. B Lymphocytes and Macrophages in the Perivascular Adipose Tissue Are Associated With Coronary Atherosclerosis: An Autopsy Study. J Am Heart Assoc. 2019;8(24):e013793. https://doi.org/10.1161/JAHA.119.013793

11. He C, Kim HI, Park J, Guo J, Huang W. The role of immune cells in different stages of atherosclerosis. Int J Med Sci. 2024;21(6):1129-1143. https://doi.org/10.7150/ijms.94570

12. Khan SR, Dalm VASH, Ikram MK, Peeters RP, van Hagen PM, et al. The Association of Serum Immunoglobulins with Risk of Cardiovascular Disease and Mortality: the Rotterdam Study. J Clin Immunol. 2023;43(4):769-779. https://doi.org/10.1007/s10875-023-01433-7