DIAGNOSTIC CHARACTERISTICS OF A LATEX ANTIGEN HELICOBACTER TEST-SYSTEM
https://doi.org/10.21886/2219-8075-2014-2-41-44
Abstract
Purpose: The study of operation diagnostic characteristics of a newly constructed helicobacter test-system which is designed to determine the total antibodies in the blood serum in volumetric agglomeration reaction.
Materials and methods: Blood sera of 224 gastroenterological patients were studied with the help of diagnostic system constructed. Commercial immune-enzyme blood assay systems were used to be the referent method.
Results: Diagnostic operation characteristics of a latex antigen helicobacter test-system were determined with the help of referent serological diagnostic methods. At the cut-off point 1:160 the sensitivity, specificity and diagnostic effectiveness are 94,9%, 79,3% и 88,2%, respectively.
Summary: The cut-off point 1:160 is shown to be used as a diagnostic threshold.
Materials and methods: Blood sera of 224 gastroenterological patients were studied with the help of diagnostic system constructed. Commercial immune-enzyme blood assay systems were used to be the referent method.
Results: Diagnostic operation characteristics of a latex antigen helicobacter test-system were determined with the help of referent serological diagnostic methods. At the cut-off point 1:160 the sensitivity, specificity and diagnostic effectiveness are 94,9%, 79,3% и 88,2%, respectively.
Summary: The cut-off point 1:160 is shown to be used as a diagnostic threshold.
About the Authors
E. A. Bereznyak
Rostov-on Don Institute for Plague Control of the Federal Agency on consumer Rights Protection and Human Welfare Supervision
Russian Federation
Russian Federation
L. M. Verkina
Rostov-on Don Institute for Plague Control of the Federal Agency on consumer Rights Protection and Human Welfare Supervision
Russian Federation
Russian Federation
Yu. L. Bereznyak
Rostov State Medical University
Russian Federation
Russian Federation
I. R. Simonova
Rostov-on Don Institute for Plague Control of the Federal Agency on consumer Rights Protection and Human Welfare Supervision
Russian Federation
Russian Federation
References
1. Барышникова, Н. В. Актуальные проблемы диагностики хеликобактериоза/Н. В.Барышникова//Экспериментальная и клиническая гастроэнтерология. -2009. -№2. -С. 50-54.
2. Mégraud, F. Helicobacter pylori Detection and Antimicrobial Susceptibility Testing/F. Mégraud, P. Lehours//Clinical Microbiology Reviews. -2007. -Vol. 20. -No. 2. -P. 280-322.
3. Жуховицкий, В. Г. «Золотой стандарт» и алгоритм микробиологической диагностики хелибактериоза/В. Г. Жуховицкий, Л. В. Диденко, Н. Д. Константинова//Клин. лаб. диагн. -2005. -№9. -С.52-53.
4. Malfertheiner, P. Guidelines for the management of Helicobacter pylori infection. Summary of the Maastricht III 2005 consensus report. Business Briefing/P. Malfertheiner, F. Megraud, C. O’Morain//European Gastroenterology Review. -2005. -P. 59-63.
5. Basinska, T. Principle of a new immunoassay based on electrophoretic mobility of poly(styrene/alpha-tert-butoxy-omega-vinylbenzyl-polyglycidol) microspheres: application for the determination of Helicobacter pylori IgG in blood serum/T. Basinska, M. Wisniewska, M. Chmiela//Macromol. Biosci. -2005. -Vol.5 (1). -P. 70-77.
6. Станишевский, Я.М. Создание диагностических тест систем «Полимерная микросфера-биолиганд» медико-биологического применения: автореф. дис…док. хим. наук: 03.01.06: -Москва, 2012.-47с.
7. Адамов, А. К., Агафонов, В. И. Суспензионные антигены, антитела и иммуносорбенты/-М.: 1969. -175 c.
8. Флетчер, Р., Флетчер С., Вагнер Э. Клиническая эпидемиология. Основы доказательной медицины. -М.:Медиасфера, 1998. -318с.
9. Graham, D. Y. Challenge model for Helicobacter pylori infection in human volunteers/D. Y. Graham, A. R. Opekun, M. S. Osata et al.//Gut. -2004. -Vol. 53. -P. 1235-1243.
10. Березняк, Е.А. Генотипирование штаммов Helicobacter pylori, циркулирующих в Ростовской области/Е.А. Березняк, В.М. Сорокин, Е.К. Гончаров, Д.И. Симакова, Л.В. Ларионова, Г.Л. Карбышев, А.Н. Терентьев//Межд. мед.-сан. правила и реализ. глобал. стратегии борьбы с инф. бол. в гос-вах-учас. СНГ. Матер. VIII Межгос. науч.-практ. конф. -Саратов, 2007. -С. 176-177.
Review
For citations:
Bereznyak E.A., Verkina L.M., Bereznyak Yu.L., Simonova I.R. DIAGNOSTIC CHARACTERISTICS OF A LATEX ANTIGEN HELICOBACTER TEST-SYSTEM. Medical Herald of the South of Russia. 2014;(2):41-44. (In Russ.) https://doi.org/10.21886/2219-8075-2014-2-41-44
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