Study of the state of hypoxia of the renal tissue in patients with oxalate urolithiasis
https://doi.org/10.21886/2219-8075-2019-10-2-22-28
Abstract
Objective: tto determine the contribution of key metabolites and enzymes, taking into account the polymorphism of the latter, in the development of oxalic urolithiasis.
Materials and methods: the study included 72 patients (30 men and 42 women) with previously confirmed oxalic urolithiasis. Blood samples were taken from patients previously divided into groups depending on the stage of treatment. Lactate, lactate dehydrogenase, protein-bound hydroxyproline, homocysteine, malondialdehyde, and glutathione peroxidase in blood were determined, the polymorphism of the MTHFR gene was studied.
Results: the change in the concentration of the studied markers in the serum of different groups of patients suffering from oxalate urolithiasis was found; differences in the polymorphism of the MTHFR gene responsible for the metabolic transformations of homocysteine in the organism of patients with oxalate urolithiasis were established.
Conclusion: one of the chains of the development of oxalate urolithiasis was studied on the example of markers of hypoxia of the renal tissue.
About the Authors
A. K. Masaltsev
V.I. Razumovsky Saratov State Medical University
Russian Federation
Competing Interests: not
Russian Federation
Alexander K. Masaltsev - graduate student, Department of Biochemistry
Competing Interests: not
V. B. Borodulin
V.I. Razumovsky Saratov State Medical University
Russian Federation
Competing Interests: not
Russian Federation
Vladimir B. Borodulin - Doctor of Medical Sciences, Professor, Head of the Department of Biochemistry
Competing Interests: not
I. A. Goroshinskaya
Rostov Research Institute of Oncology
Competing Interests: not
Irina A. Goroshinskaya - Doctor of Biological Sciences, Professor, Chief Researcher of the Laboratory for Studying the Pathogenesis of Malignant Tumors.
Rostov-on-Don
Competing Interests: not
References
1. Tarasov NI, Teplyakov AT, Malakhovich EV, Fedosova NN, Kalyuzhin VV, Pushnikova EYu. The state of lipid peroxidation, blood antioxidant protection in patients with myocardial infarction, aggravated by circulatory failure. Ter. archiv. 2002;12:12-15. (in Russ).
2. Teselkin YO. Antioxidant activity of blood plasma as a criterion for assessing the functional state of the antioxidant system of the body and the effectiveness of the use of exogenous antioxidants. Dis. ... Dr. biol. sciences. Moscow; 2003. (in Russ).
3. Belyalov F.I., Mal’ceva L.E., YAgudina R.N. Unstable Angina and Comorbidity. Sibirskij medicinskij zhurnal. 2010;97(6):70-71. (in Russ).
4. Proskurnina EV. Dudina GA, Sozarukova MM, Orlova LR, Filippova YuA, et al. Free radical neutrophil-producing function in patients with myelodysplastic syndrome: diagnosis using kinetic chemiluminescence with two-step stimulation and clinical significance. Technologies of living systems. 2018;15(2):16-27. (in Russ).
5. Vladimirov YuA (Ed.). Sources and targets of free radicals in human blood. Moscow: MAX-PRESS; 2017. (in Russ).
6. Goroshinskaya IA, Surikova EI, Shalashnaya EV, Nerodo GA, Maximova NA, et al. State of free radical processes in ovarian cancer with different prevalence and course of the disease. Izvestiya vuzov. Severo-Kavkazskii region. 2017;4-2:10-19. (in Russ). doi: 10.23683/0321-3005-2017-4-2-10-19
7. Al-Khafaji AB, Tohme S, Yazdani HO, Miller D, Huang H, Tsung A. Superoxide induces neutrophil extracellular trap formation in a TLR-4 and NOX-dependent mechanism. Molecular medicine. 2016;22. doi:10.2119/molmed.2016.00054
8. Thieblemont N, Wright HL, Edwards SW, Witko-Sarsat V Human neutrophils in auto-immunity. Seminars in immunology. 2016;28:159-173. doi: 10.1016/j.smim.2016.03.004
9. Tecchio C, Cassatella MA. Neutrophil-derived chemokines on the road to immunity. Seminars in immunology. 2016;28:119-128. doi: 10.1016/j.smim.2016.04.003
10. Nemeth T, Mocsai A, Lowell CA. Neutrophils in animal models of autoimmune disease. Seminars in immunology. 2016;28:174-186. doi: 10.1016/j.smim.2016.04.001
11. Awasthi D, Nagarkoti S, Kumar A, Dubey M, Singh AK, et al. Oxidized LDL induced extracellular trap formation in human neutrophils via TLR-PKC-IRAK-MAPK and NADPH-oxidase activation. Free radical biology & medicine. 2016;93:190-203. doi: 10.1016/j.freeradbiomed.2016.01.004
12. Minushkina LO. Angiotensin-converting enzyme genes, NOsynthetase and endothelin-1, and left ventricular myocardial hypertrophy in patients with hypertension of the indigenous people of Yakutia. Kardiologiya. 2005;1:41-45. (in Russ).
13. Miyamoto Y, Saitio Y, Kajiyama N, Yoshimura M, Shimasaki Y, et al. Endothelial nitric oxide synthase gene is positively associated with essential hypertension. Hypertension. 1998;32:3-8.
14. Heux S, Morin F, Lea RA. The methylentetrahydrofolate reductase gene variant (C677T) as a risk factor for essential hypertension in Caucasians. Hypertens. Res. 2004;27(9):663-667.
15. Hein DW, Doll MA, Fretland AJ. Molecular genetics and epidemiology of the NAT1 and NAT2 acetylation polymorphisms. Cancer Epidemiol Biomarkers Prev. 2000;9(1):29-42.
16. Vatsis KP, Weber WW, Bell DA. Nomenclature for N-acetyl-transferases. Pharmacogenetics. 1995;5(1):1-17.
17. Goldenkova-Pavlova IV, Bruskin SA, Abdeev RM. et al. Comparative analysis of the results of phenotyping and genotyp-ing on N-acetylation polymorphism in humans. Genetika: zhurnal Rossijskoj akademii nauk. 2006;42(8):1143-1150. (in Russ).
18. Pustovalova LM. Workshop on biochemistry. Rostov-on-Don: Feniks; 1999. (in Russ).
19. Stalnaya ID, Garishvili TG. Method for the determination of malonic dialdehyde using thiobarbituric acid. In: Modern methods in biochemistry, Ed. V.N. Orekhovich. M.: Medicine; 1977. P. 66-68. (in Russ).
20. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967;70:158-169.
21. Glanz S. Biomedical statistics. M.: Praktika; 1998. (in Russ).
22. Shevchenko O.P., Olefirenko G.A., Chervyakova N.V. Homocysteine. Moscow; 2002. (in Russ).
23. Goroshinskaya I.A., Shevchenko A.N., Filatova E.V., Kachesova P.S., Nemashkalova L.A., Chudilova A.V. Effect of intravesical chemotherapy, modified by electromagnetic field in the scanning mode, on the level of endotoxemia and oxidative processes in the blood of bladder cancer patients. Ros-sijskij onkologicheskij zhurnal. 2017;22(3):142-148. (in Russ).
Review
For citations:
Masaltsev A.K., Borodulin V.B., Goroshinskaya I.A. Study of the state of hypoxia of the renal tissue in patients with oxalate urolithiasis. Medical Herald of the South of Russia. 2019;10(2):22-28. (In Russ.) https://doi.org/10.21886/2219-8075-2019-10-2-22-28
Views:
817
Email this article 