THE ROLE OF EXPRESSION OF TLR2, TLR6 AND POLYMORPHISM OF THEIR GENES IN THE DEVELOPMENT OF GENERALIZED CYTOMEGALOVIRUS AND HERPETIC INFECTIONS IN NEWBORNS
https://doi.org/10.21886/2219-8075-2015-3-24-27
Abstract
Purpose: identification of new mechanisms of the development of generalized herpes virus infection (current cytomegalovirus infections and infections caused HSV1,2 types) in newborns.
Materials and Мethods: the data reviewed 69 newborns with generalized herpes virus infection in the early neonatal period. I group (n=47) amounted to children with generalized cytomegalovirus infection. In the second group (n=22) were included
children with generalized form of the infection caused by the herpes virus 1,2 type. The control group consisted of 26 healthy newborns. The definition of the expression of TLR-2(СD14+СD282+) and TLR-6(СD14+СD286) on monocytes conducted by laser flow cytofluorometry (Beckman Coulter, USA). Polymorphism of allelic variants of genes TLR studied by PCR and subsequent restriction fragments length polymorphism analysis test systems for molecular genetic analysis, Moscow.
Results: the decrease of expression of TLR-2 and TLR-6 has a place in generalized the current cytomegalovirus infections in children and adults with complete clinical symptoms. Newborns with severe herpes infection, there is a decrease in the expression
of TLR-thefact in the genes TLR are identified by 68.4 % of children with generalized the current cytomegalovirus infections with full clinical symptoms in the form of polymorphism Ser249Pro gene TLR-6 and 26.3% of children with severe herpes infection in the form of polymorphism Arg753Gln gene TLR-2.
Summary: the new data confirm the role of immune system in the pathogenesis of generalized herpes infection. It is established, that in children with congenital generalized herpes virus infection leading to the formation of an immune response is a breach in the system of factors of innate immunity.
About the Authors
A. A. Afonin
Rostov research Institute of obstetrics and Pediatrics
Russian Federation
Russian Federation
A. Y. Levkovich
Rostov research Institute of obstetrics and Pediatrics
Russian Federation
Russian Federation
M. A. Levkovich
Rostov research Institute of obstetrics and Pediatrics
Russian Federation
Russian Federation
L. V. Kravchenko
Rostov research Institute of obstetrics and Pediatrics
Russian Federation
Russian Federation
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Review
For citations:
Afonin A.A., Levkovich A.Y., Levkovich M.A., Kravchenko L.V. THE ROLE OF EXPRESSION OF TLR2, TLR6 AND POLYMORPHISM OF THEIR GENES IN THE DEVELOPMENT OF GENERALIZED CYTOMEGALOVIRUS AND HERPETIC INFECTIONS IN NEWBORNS. Medical Herald of the South of Russia. 2015;(3):24-27. (In Russ.) https://doi.org/10.21886/2219-8075-2015-3-24-27
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