Immunological features of oligomenorrhea in adolescent girls with excess weight

Abstract

Objective: identification of immunological features of oligomenorrhea in adolescent girls with excess weight. Materials and methods: the object of the study were 80 adolescent girls with excess weight and oligomenorrhea, who made up group 1, group 2 included 64 patients with obesity without cycle disorders. The control group — 25 healthy patients. Determination of the content of leptin, sR-leptin, IL-6, TNF-α, sTNF-αR1 was carried out by the method of solid-phase enzyme immunoassay (BenderMedSystems). Expression of TLR -2, TLR-4 was analyzed by flow cytometry (HyCultbiotechnology). Analysis of nucleotide polymorphism of the TLR-4 Asp299Gly gene was performed by PCR using the standard kit of the State Research Institute of Genetics (Russia). Descriptive statistics were performed using the Mann-Whitney criterion, with an error of the first kind p <0.05. To study the frequency of allele and genotype polymorphism by a binary feature, the χ2 criterion was used. Results: chronic inflammation is associated with metabolic disorders, dysregulation of carbohydrate and lipid metabolism. Oligomenorrhea in overweight patients is associated with an increase in the production of proinflammatory cytokines, the presence of mutations in the TLR-4 gene. Conclusions: the established changes potentiate ovarian dysfunction and the development of oligomenorrhea.