mvjrМедицинский вестник Юга РоссииMedical Herald of the South of Russia2219-80752618-7876The Rostov State Medical University10.21886/2219-8075-2018-9-1-51-62mvjr-636Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПредиктивная диагностика метастатического поражения лимфоузлов у больных аденокарциномой желудкаPredictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinomaКитО. И.KitO. I.

член-корр. РАН, д.м.н., профессор, генеральный директор

corresponding member. RAS, Doctor of Medicine, Professor, General Director

onko-sekretar@mail.ruhttps://orcid.org/0000-0002-8942-3733КутилинД. С.KutilinD. S.

к.б.н., с.н.с. лаборатории молекулярной онкологии,SPIN-код: 8382-4460, AuthorID: 794680, Scopus Author ID: 55328886800

Ph.D. Laboratory of Molecular Oncology, Rostov Research Institute of Oncology

k.denees@yandex.ruТатимовМ. З.TatimovM. Z.

аспирант

post-graduate student

tatim4ik07@mail.ruМасловА. А.MaslovA. A.

доктор медицинских наук, профессор, главный врач

MD, Professor, Chief Physician

maslovaa@rnioi.ruФранциянцЕ. М.FrantsiyantsE. M.

д.б.н., профессор, заместитель генерального директора по науке

Doctor of Biological Sciences, Professor, Deputy Director General for Science

super.gormon@yandex.ruВодолажскийД. И.VodolazhskyD. I.

к.б.н., руководитель лаборатории молекулярной онкологии

Ph.D., Head of the Laboratory of Molecular Oncology

dvodolazhsky@gmail.comФГБУ "Ростовский научно-исследовательский онкологический институт" МЗ РФ, Ростов-на-ДонуРоссияRostov Research Institute of Oncology, Rostov-on-DonRussian FederationФГБУ "Ростовский научно-исследовательский онкологический институт" МЗ РФРоссияRostov Research Institute of Oncology, Rostov-on-DonRussian Federation201823032018915162Copyright © Кит О.И., Кутилин Д.С., Татимов М.З., Маслов А.А., Франциянц Е.М., Водолажский Д.И., 20182018Кит О.И., Кутилин Д.С., Татимов М.З., Маслов А.А., Франциянц Е.М., Водолажский Д.И.Kit O.I., Kutilin D.S., Tatimov M.Z., Maslov A.A., Frantsiyants E.M., Vodolazhsky D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medicalherald.ru/jour/article/view/636

Objective: lymph node metastases occur in 90% of cases in patients with gastric adenocarcinoma, and survival is less than 15% in the late stages of disease. Therefore, the purpose of the study was to screening markers for predicting the likelihood of metastases developing in gastric adenocarcinoma. Materials and methods: for the study 47 patients with diagnosis of gastric adenocarcinoma were used (with the localized tumor form T3-4N0M0 (n = 18) and the locally distributed form T3-4N1-3M0 (n = 29)). The copy number variation of 16 genes (APC, AURKA, CCND1, C-MYC, GKN1, HER2, IRX1, MDM2, MET, NFKB, OCT1, P53, PIK3CA, POU5F1B, S6K2 and HV2) was determined by RT-PCR. Immunohistochemical studies were performed on sections of paraffin blocks using monoclonal antibodies to p53, Bcl-2, Ki-67 and Her2. Results: the loss of duplicity of HV2 locus was most frequent CNV in samples of stomach adenocarcinoma, but CNV significant change in the T3-4N0M0 group relative to T3-4N1-3M0 was not detected. A significant increase of CNV in the T3-4N1-3M0 group relative to T3-4N0M0 was found in 2 of 16 loci - NFKB and HER2 in 1.75 and 1.90 times (p<0.0005), respectively. Th e value of p53 expression in the tumor cells nuclei in the group without metastases was lower by 52% compared to the expression in the metastatic group (p<0.05). Low proliferative activity prevailed in the group without metastases in contrast to the group with metastases, in which Her2 overexpression was also observed in 43% of cases in contrast to 20% of cases in the group without metastases. Conclusions: it was showed that the relative copies number of NFKB1 and HER2 in combination with the immunohistochemical parameters of Ki67 and HER2 in gastric tumors can be a prognostic marker of metastasis, and algorithm for predicting metastases development in gastric adenocarcinoma is formed on basis of this.

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The authors declare that there are no conflicts of interest present.