mvjrМедицинский вестник Юга РоссииMedical Herald of the South of Russia2219-80752618-7876The Rostov State Medical University10.21886/2219-8075-2017-8-4-68-73mvjr-553Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОценка генов-кандидатов в развитии сочетания гиперпластических процессов эндометрия и миомы маткиEstimation of candidate genes in the development of a combination of endometrial hyperplasia and uterine fi broidshttps://orcid.org/0000-0002-5652-0166ПономаренкоИ. В.PonomarenkoIrina V.

к.м.н, доцент кафедры медико-биологических дисциплин;

Россия, 308015, г. Белгород, ул. Победы, 85.

MD, associate professor of the Department of Biomedical Disciplines of the Medical Institute;

85 Pobedy str., Belgorod, 308015, Russia

ponomarenko_i@bsu.edu.ruhttps://orcid.org/0000-0003-1254-6134ЧурносовМ. И.ChurnosovMikhail I.

д.м.н., профессор, заведующий кафедрой медико-биологических дисциплин;

Россия, 308015, г. Белгород, ул. Победы, 85

MD, Professor, Head of the Department of Biomedical Disciplines Medical Faculty;

85 Pobedy str., Belgorod, 308015, Russia

churnosov@bsu.edu.ruБелгородский государственный национальный исследовательский университетРоссияBelgorod State National Research UniversityRussian Federation201730122017846873Copyright © Пономаренко И.В., Чурносов М.И., 20172017Пономаренко И.В., Чурносов М.И.Ponomarenko I.V., Churnosov M.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medicalherald.ru/jour/article/view/553

Цель: биоинформатическое исследование вовлеченности шести генетических полиморфизмов (rs1782507, rs555621, rs7753051, rs887912, rs6732220, rs4986938) в формирование сочетания гиперпластических процессов эндометрия с миомой матки у женщин Центрального региона России. Материалы и методы: группу исследования составили 1151 человек, из них 170 пациенток, имеющих сочетание миомы матки с гиперпластическими процессами эндометрия, и 981 женщина контрольной группы без пролиферативных заболеваний органов малого таза. Материалом для исследования послужили образцы ДНК, выделенные из цельной венозной крови методом фенольно-хлороформной экстракции.Анализ изучаемых полиморфизмов осуществляли методом детекции Taq-Man зондов с помощью real-time ПЦР.Результаты: факторами риска развития сочетания гиперпластических процессов эндометрия и миомы матки являются комбинации молекулярно-генетических маркеров С rs673220, А rs4986938 и G rs887912 (ОR=1,70), С rs6732220, А rs555621, G rs887912 (ОR=1,53) и С rs1782507 с T rs7753051 и G rs887912 (ОR=1,40). Выводы: Полученные данные свидетельствуют о значимом вкладе сочетаний полиморфных вариантов rs1782507, rs555621, rs7753051, rs887912, rs6732220 и rs4986938 в формирование сочетания гиперпластических процессов эндометрия и миомы матки среди женщин Центрального региона России.

Objective: the study of bioinformatics by six genetic polymorphisms (rs1782507, rs555621, rs7753051, rs887912, rs6732220, rs4986938) in the formation of a combination of endometrial hyperplasia with uterine myoma in women in the Central region of Russia. Materials and methods: the study group consisted of 1151 employees, including 170 patients with a combination of uterine fi broids with endometrial hyperplastic processes and 981 women in the control group without proliferative diseases of the pelvic organs. Material for the study were DNA samples isolated from venous blood by phenol-chloroform extraction.Analysis of the investigated polymorphisms was carried out by the detection ATK-man probes using real-time PCR. Results: risk factors for the development of a combination of endometrial hyperplasia and uterine fi broids combination of molecular genetic markers with rs673220, and rs4986938 and G rs887912 (ОR = 1.70), with rs6732220 and rs555621, g rs887912 (ОR = 1.53) and C rs1782507 T rs7753051 and rs887912 G (ОR = 1.40). Conclusion: the obtained data testify to the signifi cant contribution of combinations of polymorphic variants rs1782507, rs555621, rs7753051, rs887912, rs6732220 and rs4986938 to the formation of a combination of endometrial hyperplasia and uterine fi broids among women in the Central region of Russia.

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The authors declare that there are no conflicts of interest present.