mvjrМедицинский вестник Юга РоссииMedical Herald of the South of Russia2219-80752618-7876The Rostov State Medical University10.21886/2219-8075-2026-17-2-64-70mvjr-2176Research ArticleЭНДОКРИНОЛОГИЯENDOCRYNOLOGYФенотипы ожирения: вероятные маркеры, ассоциированные с микробиомом кишечникаObesity phenotypes: potential markers associated with the gut microbiomehttps://orcid.org/0000-0002-3381-9894ГаненкоЛ. А.GanenkoL. A.

Ганенко Лилия Александровна , к.м.н., доцент кафедры внутренних болезней №3

Ростов-на-Дону

Lilia A. Ganenko, Cand. Sci. (Med.), Associate Professor of the Department of Internal Medicine N 3

Rostov-on-Don

ganenko.lilia@yandex.ruhttps://orcid.org/0000-0003-4874-7835ВолковаН. И.VolkovaN. I.

Волкова Наталья Ивановна, д.м.н., профессор, заведующая кафедрой внутренних болезней №3

Ростов-на-Дону

Natalya I. Volkova, Dr. Sci. (Med.), Professor, Head of Department of internal diseases No3

Rostov-on-Don

n_i_volkova@mail.ruhttps://orcid.org/0000-0002-1428-7706ШестопаловА. В.ShestopalovA. V.

Шестопалов Александр Вячеславович , д.м.н., проф., директор

Москва

Alexander V. Shestopalov, Dr. Sci. (Med.), Professor, Director, Department of postgraduate education, residency, postgraduate studies

Moscow

al-shest@yandex.ruhttps://orcid.org/0000-0002-0937-4573НабокаЮ. Л.NabokaYu. L.

Набока Юлия Лазаревна, д.м.н., профессор, заведующая кафедрой микробиологии и вирусологии N1

Ростов-на-Дону

Yulia L. Naboka, Dr. Sci. (Med.), Professor, Head, Department of microbiology and virology N 1

Rostov-on-Don

nagu22@mail.ruРостовский государственный медицинский университетРоссияRostov State Medical UniversityRussian FederationНациональный медицинский исследовательский центр детской гематологии, онкологии и иммунологии имени Дмитрия РогачеваРоссияDmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and ImmunologyRussian Federation2026220620261726470Copyright © Ганенко Л.А., Волкова Н.И., Шестопалов А.В., Набока Ю.Л., 20262026Ганенко Л.А., Волкова Н.И., Шестопалов А.В., Набока Ю.Л.Ganenko L.A., Volkova N.I., Shestopalov A.V., Naboka Y.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medicalherald.ru/jour/article/view/2176Цель

Цель: исследовать особенности состава микробиома кишечника на уровне некоторых видов микроорганизмов в кишечнике методом NGS-секвенирования бактериальной ДНК по гену 16S рРНК у пациентов с разными метаболическими фенотипами ожирения

Материалы и методы

Материалы и методы: обследованы 95 пациентов (17 мужчин и 78 женщин, средний возраст — 50,4±4,3 лет). Сформированы две группы: I группа (n=40) — пациенты с метаболически здоровым ожирением (МЗО), II группа (n=55) — пациенты с метаболически нездоровым ожирением (МНЗО). Микробиом кишечника изучался с помощью метагеномного анализа. Секвенирование вариабельного участка v3-v4 гена 16S рРНК проводили из образцов фекалий после выделения ДНК.

Результаты

Результаты: обнаружены статистически значимые (p<0,05) различия количественных показателей некоторых видов микроорганизмов в толстой кишке у пациентов с метаболически здоровым ожирением и пациентов с метаболически нездоровым ожирением. У пациентов с МЗО количество F. prausnitzii в микробиоме кишечника выше по сравнению с МНЗО.

Выводы

Выводы: микробиом кишечника пациентов с МЗО и МНЗО имеет отличия на уровне видов некоторых таксонов микроорганизмов.

Objective

Objective: to study the compositional features of the intestinal microbiome at the level of certain microbial species in the intestine using NGS sequencing of bacterial DNA for the 16S rRNA gene in patients with different metabolic phenotypes of obesity.

Materials and methods

Materials and methods: ninety-five patients (17 men and 78 women, mean age 50.4±4.3 years) were examined. Two groups were formed: group 1 (n=40) — patients with metabolically healthy obesity (MHO), group 2 (n=55) — patients with metabolically unhealthy obesity (MUHO). The intestinal microbiome was studied using metagenomic analysis. Sequencing of the v3-v4 variable region of the 16S rRNA gene was performed from fecal samples after DNA isolation.

Results

Results: statistically significant (p<0.05) differences in the quantitative indices of certain microbial species in the colon were found between patients with metabolically healthy obesity and those with metabolically unhealthy obesity. Patients with metabolically healthy obesity had higher levels of F. prausnitzii in their intestinal microbiomes compared to those with metabolically unhealthy obesity.

Conclusions

Conclusions: the intestinal microbiomes of patients with metabolically healthy obesity and those with metabolically unhealthy obesity differed at the species level for certain microbial taxa.

метаболически здоровое ожирениеметаболически нездоровое ожирениемикробиом кишечникавиды микроорганизмовметагеномное секвенированиеmetabolically healthy obesitymetabolically unhealthy obesitygut microbiomemicrobial speciesmetagenomic sequencingРабота выполнена в рамках договора №0373100122119000041 по проекту «Создание банка биообразцов сыворотки крови и фекалий от здоровых доноров и пациентов с ожирением, метаболическим синдромом, сахарным диабетом 2 типа, нарушением мукозального барьера желудочно-кишечного тракта с целью выявления кандидатных видонеспецифических медиаторов систем quorum sensing микробиоты человека, модулирующих эндокринную и метаболическую функцию жировой ткани».The work was carried out within the framework of the contract No. 0373100122119000041 for the project “Creation of a bank of blood serum and feces biosamples from healthy donors and patients with obesity, metabolic syndrome, type 2 diabetes mellitus, and impaired mucosal barrier of the gastrointestinal tract in order to identify candidate species-nonspecific mediators of human microbiota quorum sensing systems that modulate the endocrine and metabolic function of adipose tissue”.ReferencesPhillips CM. 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The authors declare that there are no conflicts of interest present.