mvjrМедицинский вестник Юга РоссииMedical Herald of the South of Russia2219-80752618-7876The Rostov State Medical University10.21886/2219-8075-2025-16-3-67-74mvjr-2129Research ArticleЭНДОКРИНОЛОГИЯENDOCRYNOLOGYВозможные маркеры ожирения, связанные с микробиомом кишечникаPotential markers of obesity linked to the gut microbiomehttps://orcid.org/0000-0002-3381-9894ГаненкоЛ. А.GanenkoL. A.

Ганенко Лилия Александровна, к.м.н., доцент кафедры внутренних болезней № 3

Ростов-на-Дону

Lilia A. Ganenko, Cand. Sci. (Med.), Associate Professor of the Department of Internal Medicine N 3

Rostov-on-Don

ganenko.lilia@yandex.ruhttps://orcid.org/0000-0003-4874-7835ВолковаН. И.VolkovaN. I.

Волкова Наталья Ивановна, д.м.н., проф., заведующая кафедрой внутренних болезней № 3

Ростов-на-Дону

Natalya I. Volkova, Dr. Sci. (Med.), Professor, Head of Department of internal diseases No 3

Rostov-on-Don

n_i_volkova@mail.ruhttps://orcid.org/0000-0002-1428-7706ШестопаловА. В.ShestopalovA. V.

Александр Вячеславович Шестопалов, д.м.н., проф., зам. директора Центра цифровой и трансляционной биомедицины «Центр молекулярного здоровья»; директор управления последипломного образования, ординатуры, аспирантуры НМИЦ ДГОИ им. Дмитрия Рогачева

Москва

Alexander V. Shestopalov, D. Sci. (Med.), Professor, Deputy director, Center of Digital and Translational Biomedicine, Center for Molecular Health; Director, Department of postgraduate education, residency, postgraduate studies, Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology; Head, Department of biochemistry and molecular biology, Medical faculty, N.I. Pirogov Russian National Research Medical University

Moscow

al-shest@yandex.ruhttps://orcid.org/0000-0002-0937-4573НабокаЮ. Л.NabokaYu. L.

Набока Юлия Лазаревна, д.м.н., проф., заведующая кафедрой микробиологии и вирусологии № 1

Ростов-на-Дону

Yulia L. Naboka, D. Sci. (Med.), Professor, Head, Department of microbiology and virology N 1

Rostov-on-Don

nagu22@mail.ruhttps://orcid.org/0009-0003-8219-395XЗибаревА. Л.ZibarevA. L.

Зибарев Александр Леонидович, ассистент кафедры внутренних болезней № 3

Ростов-на-Дону

Alexander L. Zibarev, Assistant Professor of the Department of Internal Medicine N 3

Rostov-on-Don

zibarev_al@mail.ruРостовский государственный медицинский университетРоссияRostov State Medical UniversityRussian FederationНациональный медицинский исследовательский центр детской гематологии, онкологии и иммунологии им. Д. РогачеваРоссияDmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and ImmunologyRussian Federation2025250920251636774Copyright © Ганенко Л.А., Волкова Н.И., Шестопалов А.В., Набока Ю.Л., Зибарев А.Л., 20252025Ганенко Л.А., Волкова Н.И., Шестопалов А.В., Набока Ю.Л., Зибарев А.Л.Ganenko L.A., Volkova N.I., Shestopalov A.V., Naboka Y.L., Zibarev A.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medicalherald.ru/jour/article/view/2129Цель

Цель: изучить особенности содержания некоторых видов микроорганизмов в толстой кишке методом метагеномного секвенирования у пациентов с ожирением и у здоровых людей с нормальной массой тела.

Материалы и методы

Материалы и методы: обследованы 265 человек (44 мужчин и 221 женщина, средний возраст — 47,1 ± 4,8 года). Сформированы две клинические группы: здоровые с нормальной массой тела (n = 129) и пациенты с ожирением (n = 136). Оценка состава микробиома кишечника выполнена путём метагеномного анализа. Из образцов кала выделяли ДНК и проводили секвенирование вариабельного участка v3–v4 гена 16S рРНК.

Результаты

Результаты: выявлены статистически значимые (p < 0,05) различия количественных и качественных показателей некоторых видов микроорганизмов в толстой кишке у здоровых людей без ожирения и у пациентов с ожирением. У пациентов с ожирением повышено количество P. copri. и снижено количество F. prausnitzii, B. adolescentis и B. longum.

Выводы

Выводы: полученные результаты продемонстрировали определённые видовые различия в микробиоме кишечника у здоровых лиц и у пациентов с ожирением.

Objective: to study the features of the content of some types of microorganisms in the colon in obese patients and healthy individuals with normal body weight using metagenomic sequencing. Materials and methods: a total of 265 people (44 men and 221 women, average age 47.1±4.8 years) were examined. Two clinical groups were formed: healthy individuals with normal body weight (n=129) and obese patients (n=136). The composition of the intestinal microbiome was assessed using metagenomic analysis. DNA was isolated from fecal samples and sequencing of the v3-v4 variable region of the 16S rRNA gene was performed. Results: statistically significant (p<0.05) differences in the quantitative and qualitative parameters of some types of microorganisms in the colon of healthy individuals without obesity and in obese patients were revealed. The number of P. copri was increased in obese patients. and decreased F. prausnitzii, B. adolescentis and B. longum. Conclusions: the obtained results demonstrated certain species differences in the intestinal microbiome in healthy individuals and in obese patients.

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The authors declare that there are no conflicts of interest present.