mvjrМедицинский вестник Юга РоссииMedical Herald of the South of Russia2219-80752618-7876The Rostov State Medical University10.21886/2219-8075-2023-14-3-66-72mvjr-1747Research Article3.1.22 ИНФЕКЦИОННЫЕ БОЛЕЗНИ3.1.22 INFECTIOUS DISEASESПрофилактическая эффективность препаратов везикул наружных мембран атоксигенных штаммов Vibrio cholerae О1 серогруппыPreventive efficacy of preparations of vesicles of external membranes of atoxygenic strains of Vibrio cholerae О1 serogrouphttps://orcid.org/0000-0002-1103-4244ФилиппенкоА. В.FilippenkoA. V.

Филиппенко Анна Владимировна - младший научный сотрудник лаборатории иммунологии.

Ростов-на-Дону

Anna V. Filippenko - Junior Researcher, Laboratory of Immunology, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

filippenko.annushka@mail.ruhttps://orcid.org/0000-0001-5208-7724ОмельченкоН. Д.OmelchenkoN. D.

Омельченко Наталья Дмитриевна – кандидат медицинских наук, старший научный сотрудник лаборатории иммунологии.

Ростов-на-Дону

Natalia D. Omelchenko - Cand. Sci. (Med.), Senior Researcher Laboratory of Immunology, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

natalya.omelchenko@yandex.ruhttps://orcid.org/0000-0002-1702-1620ДувановаО. В.DuvanovaO. V.

Дуванова Ольга Викторовна – кандидат биологических наук, старший научный сотрудник лаборатории микробиологии холеры и других ОКИ.

Ростов-на-Дону

Olga V. Duvanova - Cand. Sci. (Bio.), Senior Researcher Laboratory of Microbiology of Cholera and other Acute Intestinal Infections, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

olga_duvanova@mail.ruhttps://orcid.org/0000-0002-8517-2789ШипкоЕ. С.ShipkoE. S.

Шипко Елена Сергеевна - младший научный сотрудник лаборатории лаборатории микробиологии холеры и других ОКИ.

Ростов-на-Дону

Elena S. Shipko - Junior Researcher, Laboratory of Microbiology of Cholera and other Acute Intestinal Infections, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

helena.shipman@gmail.comhttps://orcid.org/0000-0002-4770-5994ТруфановаА. А.TrufanovaA. A.

Труфанова Анастасия Александровна - младший научный сотрудник лаборатории иммунологии.

Ростов-на-Дону

Anastasia A. Trufanova - Junior Researcher, Laboratory of Immunology, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

nastyasia61@mail.ruhttps://orcid.org/0000-0002-1525-5693ПасюковаН. И.PasyukovaN. I.

Пасюкова Нина Ивановна - научный сотрудник лаборатории экспериментально-биологических моделей и биологической безопасности.

Ростов-на-Дону

Nina I. Pasyukova - Researcher at the Laboratory of Experimental Biological Models and Biological Safety, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

plague@aaanet.ruhttps://orcid.org/0000-0001-7068-4071ИвановаИ. А.IvanovaI. A.

Иванова Инна Александровна - кандидат биологических наук, ведущий научный сотрудник с врио зав. лабораторией иммунологии.

Ростов-на-Дону

Inna A. Ivanova - Cand. Sci. (Bio.), Leading Researcher with Acting Head of the Laboratory Immunology, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

ivanova_ia@antiplague.ruhttps://orcid.org/0000-0001-5522-9097ЕвдокимоваВ. В.EvdokimovaV. V.

Евдокимова Вероника Вячеславовна - кандидат биологических наук, научный сотрудник лаборатории диагностических препаратов.

Ростов-на-Дону

Veronika V. Evdokimova - Cand. Sci. (Bio.), Researcher at the Laboratory of Diagnostic Drugs, Rostov-on-Don Plague Control Research Institute.

Rostov-on-Don

plague@aaanet.ruРостовский-на-Дону противочумный институт РоспотребнадзораРоссияRostov-on-Don Anti-Plague Institute of RospotrebnadzorRussian Federation2023050820231436672Copyright © Филиппенко А.В., Омельченко Н.Д., Дуванова О.В., Шипко Е.С., Труфанова А.А., Пасюкова Н.И., Иванова И.А., Евдокимова В.В., 20232023Филиппенко А.В., Омельченко Н.Д., Дуванова О.В., Шипко Е.С., Труфанова А.А., Пасюкова Н.И., Иванова И.А., Евдокимова В.В.Filippenko A.V., Omelchenko N.D., Duvanova O.V., Shipko E.S., Trufanova A.A., Pasyukova N.I., Ivanova I.A., Evdokimova V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medicalherald.ru/jour/article/view/1747

Цель исследования: оценка эффективности и целесообразности применения везикул наружных мембран атоксигенных штаммов Vibrio cholerae О1 серогруппы для профилактики экспериментальной холеры. Материалы и методы. Для получения везикул использовали атоксигенные штаммы V. cholerae О1 El Tor 18950 (ctxAВ-tcpA-) и 18780 (ctxAВ-tcpA+), выделенные из воды поверхностных водоёмов. Протективные свойства полученных препаратов оценивали на модели генерализованной формы холеры у белых мышей и модели изолированной петли тонкого кишечника взрослого кролика. Результаты: показано, что везикулы наружных мембран, выделенные из атоксигенных штаммов холерного вибриона, обладают протективным действием и препятствуют развитию инфекции у экспериментальных животных при заражении их вирулентным штаммом холеры. Наибольшую эффективность полученные препараты проявляют при парентеральном двукратном, с интервалом в семь дней, введении, предотвращая гибель всех взятых в эксперимент белых мышей и развитие патогенетических процессов в тонком кишечнике взрослых кроликов. Выводы: полученные данные свидетельствует о формировании у животных выраженной иммунной защиты от заболевания, а также о возможности использования данных структур для создания профилактических противохолерных препаратов.

Purpose: To evaluate the effectiveness and expediency of using vesicles of the outer membranes of atoxygenic strains of Vibrio cholerae O1 serogroup for the prevention of experimental cholera. Materials and methods: atoxigenic strains of V. cholerae O1 El Tor 18950 (ctxAB-tcpA-) and 18780 (ctxAB-tcpA+) isolated from the water of surface reservoirs were used to obtain vesicles. The protective properties of the obtained drugs were evaluated using a model of generalized cholera in white mice and a model of an isolated loop of the small intestine of an adult rabbit. Results: it has been shown that vesicles of the outer membranes isolated from atoxygenic strains of V. cholerae have a protective effect and prevent the development of infection in experimental animals when infected with a virulent strain of cholera. The obtained drugs are most effective when administered parenterally twice, with an interval of seven days, preventing the death of all white mice taken in the experiment and the development of pathogenetic processes in the small intestine of adult rabbits. Summary: the data obtained indicates the formation of pronounced immune protection against the disease in animals, as well as the possibility of using these structures to create preventive anti-cholera drugs.

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The authors declare that there are no conflicts of interest present.